isotype matched control antibody (R&D Systems)
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isotype matched control antibody
Isotype Matched Control Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+igg2b+isotype+control/Mouse+IgG2B+APC-conjugated+Antibody/us12613244-531-21-26
Average 93 stars, based on 103 article reviews
Isotype Matched Control Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+igg2b+isotype+control/Mouse+IgG2B+APC-conjugated+Antibody/us12613244-531-21-26
Average 93 stars, based on 103 article reviews
isotype matched control antibody - by Bioz Stars,
2026-09
93/100 stars
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Fluorescence:Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome Article Snippet: .. Fluorescence‐activated cell sorting (FACS) buffer was prepared by filtering phosphate‐buffered saline (PBS) [2.7 mM KCl (Nacalai Tesque), 1.5 mM KH 2 PO 4 (Nacalai Tesque), 137 mM NaCl (Nacalai Tesque), 18 mM Na 2 HPO 4 ·7H 2 O (Nacalai Tesque)] containing 0.5% BSA and 125 mM di‐sodium dihydrogen ethylenediaminetetraacetate (Nacalai Tesque), and blocking buffer was prepared by adding 1% mouse serum (Sigma‐Aldrich) and 1% rat serum (Sigma‐Aldrich) to the FACS buffer. mESC‐derived macrophage progenitors were detached using TrypLE (1×) for 3 min and then incubated with primary antibodies: anti‐BMPR1B (1:300, R&D Systems, MAB5051), anti‐VEGFR1 (1:8, R&D Systems, AF471), anti‐VEGFR2 (1:8, R&D Systems, AF644), anti‐VEGFR3 (1:80, R&D Systems, AF743), Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome. Article Snippet: .. Fluorescence- activated cell sorting (FACS) buffer was prepared by filtering phosphate- buffered saline (PBS) [2.7 mM KCl (Nacalai Tesque), 1.5 mM KH2PO4 (Nacalai Tesque), 137 mM NaCl (Nacalai Tesque), 18 mM Na2HPO4·7H2O (Nacalai Tesque)] containing 0.5% BSA and 125 mM di- sodium dihydrogen ethylenediaminetetraacetate (Nacalai Tesque), and blocking buffer was prepared by adding 1% mouse serum (Sigma- Aldrich) and 1% rat serum (Sigma- Aldrich) to the FACS buffer. mESCderived macrophage progenitors were detached using TrypLE (1×) for 3 min and then incubated with primary antibodies: anti- BMPR1B (1:300, R&D Systems, MAB5051), anti- VEGFR1 (1:8, R&D Systems, AF471), anti- VEGFR2 (1:8, R&D Systems, AF644), anti- VEGFR3 (1:80, R&D Systems, AF743), FACS:Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome Article Snippet: .. Fluorescence‐activated cell sorting (FACS) buffer was prepared by filtering phosphate‐buffered saline (PBS) [2.7 mM KCl (Nacalai Tesque), 1.5 mM KH 2 PO 4 (Nacalai Tesque), 137 mM NaCl (Nacalai Tesque), 18 mM Na 2 HPO 4 ·7H 2 O (Nacalai Tesque)] containing 0.5% BSA and 125 mM di‐sodium dihydrogen ethylenediaminetetraacetate (Nacalai Tesque), and blocking buffer was prepared by adding 1% mouse serum (Sigma‐Aldrich) and 1% rat serum (Sigma‐Aldrich) to the FACS buffer. mESC‐derived macrophage progenitors were detached using TrypLE (1×) for 3 min and then incubated with primary antibodies: anti‐BMPR1B (1:300, R&D Systems, MAB5051), anti‐VEGFR1 (1:8, R&D Systems, AF471), anti‐VEGFR2 (1:8, R&D Systems, AF644), anti‐VEGFR3 (1:80, R&D Systems, AF743), Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome. Article Snippet: .. Fluorescence- activated cell sorting (FACS) buffer was prepared by filtering phosphate- buffered saline (PBS) [2.7 mM KCl (Nacalai Tesque), 1.5 mM KH2PO4 (Nacalai Tesque), 137 mM NaCl (Nacalai Tesque), 18 mM Na2HPO4·7H2O (Nacalai Tesque)] containing 0.5% BSA and 125 mM di- sodium dihydrogen ethylenediaminetetraacetate (Nacalai Tesque), and blocking buffer was prepared by adding 1% mouse serum (Sigma- Aldrich) and 1% rat serum (Sigma- Aldrich) to the FACS buffer. mESCderived macrophage progenitors were detached using TrypLE (1×) for 3 min and then incubated with primary antibodies: anti- BMPR1B (1:300, R&D Systems, MAB5051), anti- VEGFR1 (1:8, R&D Systems, AF471), anti- VEGFR2 (1:8, R&D Systems, AF644), anti- VEGFR3 (1:80, R&D Systems, AF743), Saline:Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome Article Snippet: .. Fluorescence‐activated cell sorting (FACS) buffer was prepared by filtering phosphate‐buffered saline (PBS) [2.7 mM KCl (Nacalai Tesque), 1.5 mM KH 2 PO 4 (Nacalai Tesque), 137 mM NaCl (Nacalai Tesque), 18 mM Na 2 HPO 4 ·7H 2 O (Nacalai Tesque)] containing 0.5% BSA and 125 mM di‐sodium dihydrogen ethylenediaminetetraacetate (Nacalai Tesque), and blocking buffer was prepared by adding 1% mouse serum (Sigma‐Aldrich) and 1% rat serum (Sigma‐Aldrich) to the FACS buffer. mESC‐derived macrophage progenitors were detached using TrypLE (1×) for 3 min and then incubated with primary antibodies: anti‐BMPR1B (1:300, R&D Systems, MAB5051), anti‐VEGFR1 (1:8, R&D Systems, AF471), anti‐VEGFR2 (1:8, R&D Systems, AF644), anti‐VEGFR3 (1:80, R&D Systems, AF743), Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome. Article Snippet: .. Fluorescence- activated cell sorting (FACS) buffer was prepared by filtering phosphate- buffered saline (PBS) [2.7 mM KCl (Nacalai Tesque), 1.5 mM KH2PO4 (Nacalai Tesque), 137 mM NaCl (Nacalai Tesque), 18 mM Na2HPO4·7H2O (Nacalai Tesque)] containing 0.5% BSA and 125 mM di- sodium dihydrogen ethylenediaminetetraacetate (Nacalai Tesque), and blocking buffer was prepared by adding 1% mouse serum (Sigma- Aldrich) and 1% rat serum (Sigma- Aldrich) to the FACS buffer. mESCderived macrophage progenitors were detached using TrypLE (1×) for 3 min and then incubated with primary antibodies: anti- BMPR1B (1:300, R&D Systems, MAB5051), anti- VEGFR1 (1:8, R&D Systems, AF471), anti- VEGFR2 (1:8, R&D Systems, AF644), anti- VEGFR3 (1:80, R&D Systems, AF743), Blocking Assay:Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome Article Snippet: .. Fluorescence‐activated cell sorting (FACS) buffer was prepared by filtering phosphate‐buffered saline (PBS) [2.7 mM KCl (Nacalai Tesque), 1.5 mM KH 2 PO 4 (Nacalai Tesque), 137 mM NaCl (Nacalai Tesque), 18 mM Na 2 HPO 4 ·7H 2 O (Nacalai Tesque)] containing 0.5% BSA and 125 mM di‐sodium dihydrogen ethylenediaminetetraacetate (Nacalai Tesque), and blocking buffer was prepared by adding 1% mouse serum (Sigma‐Aldrich) and 1% rat serum (Sigma‐Aldrich) to the FACS buffer. mESC‐derived macrophage progenitors were detached using TrypLE (1×) for 3 min and then incubated with primary antibodies: anti‐BMPR1B (1:300, R&D Systems, MAB5051), anti‐VEGFR1 (1:8, R&D Systems, AF471), anti‐VEGFR2 (1:8, R&D Systems, AF644), anti‐VEGFR3 (1:80, R&D Systems, AF743), Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome. Article Snippet: .. Fluorescence- activated cell sorting (FACS) buffer was prepared by filtering phosphate- buffered saline (PBS) [2.7 mM KCl (Nacalai Tesque), 1.5 mM KH2PO4 (Nacalai Tesque), 137 mM NaCl (Nacalai Tesque), 18 mM Na2HPO4·7H2O (Nacalai Tesque)] containing 0.5% BSA and 125 mM di- sodium dihydrogen ethylenediaminetetraacetate (Nacalai Tesque), and blocking buffer was prepared by adding 1% mouse serum (Sigma- Aldrich) and 1% rat serum (Sigma- Aldrich) to the FACS buffer. mESCderived macrophage progenitors were detached using TrypLE (1×) for 3 min and then incubated with primary antibodies: anti- BMPR1B (1:300, R&D Systems, MAB5051), anti- VEGFR1 (1:8, R&D Systems, AF471), anti- VEGFR2 (1:8, R&D Systems, AF644), anti- VEGFR3 (1:80, R&D Systems, AF743), Incubation:Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome Article Snippet: .. Fluorescence‐activated cell sorting (FACS) buffer was prepared by filtering phosphate‐buffered saline (PBS) [2.7 mM KCl (Nacalai Tesque), 1.5 mM KH 2 PO 4 (Nacalai Tesque), 137 mM NaCl (Nacalai Tesque), 18 mM Na 2 HPO 4 ·7H 2 O (Nacalai Tesque)] containing 0.5% BSA and 125 mM di‐sodium dihydrogen ethylenediaminetetraacetate (Nacalai Tesque), and blocking buffer was prepared by adding 1% mouse serum (Sigma‐Aldrich) and 1% rat serum (Sigma‐Aldrich) to the FACS buffer. mESC‐derived macrophage progenitors were detached using TrypLE (1×) for 3 min and then incubated with primary antibodies: anti‐BMPR1B (1:300, R&D Systems, MAB5051), anti‐VEGFR1 (1:8, R&D Systems, AF471), anti‐VEGFR2 (1:8, R&D Systems, AF644), anti‐VEGFR3 (1:80, R&D Systems, AF743), Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome. Article Snippet: .. Fluorescence- activated cell sorting (FACS) buffer was prepared by filtering phosphate- buffered saline (PBS) [2.7 mM KCl (Nacalai Tesque), 1.5 mM KH2PO4 (Nacalai Tesque), 137 mM NaCl (Nacalai Tesque), 18 mM Na2HPO4·7H2O (Nacalai Tesque)] containing 0.5% BSA and 125 mM di- sodium dihydrogen ethylenediaminetetraacetate (Nacalai Tesque), and blocking buffer was prepared by adding 1% mouse serum (Sigma- Aldrich) and 1% rat serum (Sigma- Aldrich) to the FACS buffer. mESCderived macrophage progenitors were detached using TrypLE (1×) for 3 min and then incubated with primary antibodies: anti- BMPR1B (1:300, R&D Systems, MAB5051), anti- VEGFR1 (1:8, R&D Systems, AF471), anti- VEGFR2 (1:8, R&D Systems, AF644), anti- VEGFR3 (1:80, R&D Systems, AF743), Control:Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome Article Snippet: .. Fluorescence‐activated cell sorting (FACS) buffer was prepared by filtering phosphate‐buffered saline (PBS) [2.7 mM KCl (Nacalai Tesque), 1.5 mM KH 2 PO 4 (Nacalai Tesque), 137 mM NaCl (Nacalai Tesque), 18 mM Na 2 HPO 4 ·7H 2 O (Nacalai Tesque)] containing 0.5% BSA and 125 mM di‐sodium dihydrogen ethylenediaminetetraacetate (Nacalai Tesque), and blocking buffer was prepared by adding 1% mouse serum (Sigma‐Aldrich) and 1% rat serum (Sigma‐Aldrich) to the FACS buffer. mESC‐derived macrophage progenitors were detached using TrypLE (1×) for 3 min and then incubated with primary antibodies: anti‐BMPR1B (1:300, R&D Systems, MAB5051), anti‐VEGFR1 (1:8, R&D Systems, AF471), anti‐VEGFR2 (1:8, R&D Systems, AF644), anti‐VEGFR3 (1:80, R&D Systems, AF743), Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome. Article Snippet: .. Fluorescence- activated cell sorting (FACS) buffer was prepared by filtering phosphate- buffered saline (PBS) [2.7 mM KCl (Nacalai Tesque), 1.5 mM KH2PO4 (Nacalai Tesque), 137 mM NaCl (Nacalai Tesque), 18 mM Na2HPO4·7H2O (Nacalai Tesque)] containing 0.5% BSA and 125 mM di- sodium dihydrogen ethylenediaminetetraacetate (Nacalai Tesque), and blocking buffer was prepared by adding 1% mouse serum (Sigma- Aldrich) and 1% rat serum (Sigma- Aldrich) to the FACS buffer. mESCderived macrophage progenitors were detached using TrypLE (1×) for 3 min and then incubated with primary antibodies: anti- BMPR1B (1:300, R&D Systems, MAB5051), anti- VEGFR1 (1:8, R&D Systems, AF471), anti- VEGFR2 (1:8, R&D Systems, AF644), anti- VEGFR3 (1:80, R&D Systems, AF743), Article Title: IL-21-reprogrammed Vδ1 T cells exert killing against solid tumors which is enhanced by CAR arming for off-the-shelf immunotherapy Article Snippet: .. The isotype controls used were mouse IgG1 isotype control (R&D systems) and Article Title: BMP9 knockout impairs pulmonary vessel muscularisation and confers aberrant tamoxifen sensitivity. Article Snippet: .. Administration of BMP9 monoclonal antibody Wild type C57/Bl6 mice were administered weekly for 3-weeks with 5 mg/kg BMP9 antibody (R&D Systems, Abingdon, Oxon, UK) or 5 mg/kg Article Title: IL-21-reprogrammed Vδ1 T cells exert killing against solid tumors which is enhanced by CAR arming for off-the-shelf immunotherapy Article Snippet: .. The isotype controls used were mouse IgG1 isotype control (R&D systems) and Article Title: Generation of RNA aptamers against chikungunya virus E2 envelope protein Article Snippet: The cells were incubated for 3 or 5 days depending on assays, and luciferase activity was measured using the Bright-Glo luciferase assay system (Promega, WI, USA), normalized to cell viability determined by cell counting kit-8 (Dojindo, Kumamoto, Japan) immediately before harvesting and producing relative luciferase activity. .. Anti-Chikungunya E2 protein monoclonal antibody CHK152 (Antibody Research, MO, USA) as a neutralizing antibody and Article Title: BMP9 knockout impairs pulmonary vessel muscularisation and confers aberrant tamoxifen sensitivity Article Snippet: .. Wild type C57/Bl6 mice were administered weekly for 3-weeks with 5 mg/kg BMP9 antibody (R&D Systems, Abingdon, Oxon, UK) or 5 mg/kg Article Title: Generation of RNA aptamers against chikungunya virus E2 envelope protein. Article Snippet: Nucleic acid aptamers are a promising drug modality, whereas the generation of virus-neutralizing aptamers has remained difficult due to the lack of a robust system for targeting the viral particles of interest.. Here, we took advantage of our latest platform technology of Systematic Evolution of Ligands by EXponential enrich ment (SELEX) with virus-like particles (VLPs) and targeted chikungunya virus (CHIKV) as a model, the pathogenic reemerging virus with an unmet need for control.. The identified aptamer against CHIKV-VLPs, Apt#1, and its truncated derivatives showed neutralizing activity with nanomolar IC50 values in a cell-based assay system using a pseudoviral particle of CHIKV (CHIKVpp). |
